Genomic fingerprinting ofClostridium difficileisolates by using a random amplified polymorphic DNA (RAPD) assay
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چکیده
منابع مشابه
Random amplified polymorphic DNA (RAPD) fingerprinting of Mycoplasma gallisepticum isolates from chickens
Ten field isolates of Mycoplasma gallisepticum (MG) from different geographical areas of Iran were analyzed by random amplification of polymorphic DNA (RAPD). RAPD analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. MG isolates compared with reference strains (S6 and Mg SS) and vaccine strain (ts-11) and demonstrated...
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Due to advances in molecular biology techniques, large numbers of highly informative DNA markers have been developed for the identification of genetic polymorphism. In the last decade, the random amplified polymorphic DNA (RAPD) technique based on the polymerase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA markers. RAPD markers are amplificatio...
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Construction of a genetic linkage map is necessary to apply marker-assisted selection tools in a crop improvement program. Except for the recent studies from two laboratories, most of the previous studies have shown little or no DNA polymorphism in cultivated groundnut (Arachis hypogaea L.). In the present study, 70 selected genotypes, representing variability for several morphological, physiol...
متن کاملReproducible DNA fingerprinting with the random amplified polymorphic DNA (RAPD) method.
Much interest has recently arisen in methods for DNA fingerprinting based on the polymerase chain reaction (PCR). Among these, the Random Amplified Polymorphic DNA (RAPD) method, developed by Williams etal. (1), is currently receiving particular attention (2) because of its extreme simplicity and requirement for minimal amounts of genomic DNA. The basic strategy involves the PCR amplification o...
متن کاملrandom amplified polymorphic dna (rapd) fingerprinting of mycoplasma gallisepticum isolates from chickens
ten field isolates of mycoplasma gallisepticum (mg) from different geographical areas of iran were analyzed by random amplification of polymorphic dna (rapd). rapd analysis produced reproducible banding patterns on the basis of which various distinct amplification patterns could be detected. mg isolates compared with reference strains (s6 and mg ss) and vaccine strain (ts-11) and demonstrated d...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1993
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1993.tb06567.x